联系方式
Tele: 13601585491
Email: yanxin@njau.edu.cn
教育背景
2000.9-2004.6，best365在线官网登录入口，理学学士
2004.9-2008.12，best365在线官网登录入口，微生物学博士
工作经历
2009.4-2011.12，best365在线官网登录入口生科院微生物学系，讲师
2012.1-2016.12，best365在线官网登录入口生科院微生物学系，副教授
2017.1-，best365在线官网登录入口生科院微生物学系，教授、博导
2014.8-2015.8，University of Washington（UW），访问学者
学术兼职
中国微生物学会普通微生物学专业委员会委员
中国生物工程学会一碳生物技术专业委员会委员
江苏省微生物学会青年工作委员会委员
奖励
（1）2017年“大北农青年学者奖”
（2）获863“青年科学家专题”资助
教学情况
主讲“微生物学“、“基础微生物学”和“硕士生研究班讨论”。
研究方向
（1）微生物降分解农药等有毒污染物的分子机制及微生物修复
（2）芽孢杆菌重组蛋白表达系统的开发及酶制剂生产
（3）好氧嗜甲烷菌同化甲烷的分子机理及应用
已主持的项目
（1）国家基金面上：甲烷氧化菌介导的氨氧化作用的分子机制（32270127），2023.01-2026.12
（2）国家基金面上：细菌代谢二甲酚的分子机制研究（31970099），2020.01-2023.12
（3）国家基金面上：MmoD和MmoG在sMMO合成中的作用及机理（31770125），2018.1-2021.12
（4）国家基金面上：菌株Rhodococcus sp. YL-1降解杀虫剂噻嗪酮的途径及其分子机制（31470225），2015.1-2018.12
（5）国家基金青年：基于基因组测序的苯基脲类除草剂的细菌降解分子机理研究（31000060），2011.1-2013.12
（6）863“青年科学家专题”：基于新型表达模块的芽孢杆菌表达平台的开发与应用（SS2014AA020543），2014.1-2016.12
（7）国家重点研发：塑料高效生物解聚的关键技术（2021YFC2103600），2021.12-2024.11（课题骨干）
（8）国家重点研发：人造蛋白质合成的细胞设计构建及应用（2019YFA0905200），2020.1-2024.12（课题骨干）
（9）国家重点研发：稻田典型农药多界面迁移规律及生源要素耦合的生物转化机制（2016YFD0800203），2017.1-2020.12（课题骨干）
（10）江苏省基金：稻田土著细菌降解噻嗪酮的途径及关键降解酶基因（BK20141366），2014.7-2016.7。
（11）江苏省支撑：太湖地区稻田土壤中DDT和噻嗪酮等农药污染的微生物修复关键技术应用与示范（BE2009670），2009.7-2012.7
（12）横向课题：枯草芽孢杆菌无抗基因整合及高效表达，2014-2016。
代表性论文（*通讯作者）
[1] Minggen Cheng, Dongmei Pei, Lian He, Qiang Fei, Xin Yan*. Cre/lox-Mediated CRISPRi Library Reveals Core Genome of a Type I Methanotroph Methylotuvimicrobium buryatense 5GB1C. Appl Environ Microbiol. 2023; 89(1):e0188322.
[2] Panpan Wu, Qing Tao, Yuxuan Liu, Caiting Zeng, Yu Li, Xin Yan*. Efficient secretion of mussel adhesion proteins using a chaperone protein Spy as fusion tag in Bacillus subtilis. Biotechnol J. 2023:e2200582.
[3] Bin Ye, Qing Tao, Xin Yan*. A transposon system for random insertion of a gene expression cassette into the chromosome of Bacillus subtilis. J Biotechnol. 2023; 361:66-73.
[4] Junbin Ji, Caiting Zeng, Panpan Wu, Yuying Wang, Xueting Chen, Xin Yan*. Improved Whole-Cell Biocatalyst for the Synthesis of Vitamin E Precursor 2,3,5-Trimethylhydroquinone. J Agric Food Chem. 2023;71(2):1162-1169.
[5] Junbin Ji , Minggen Cheng, Xin Yan*. Characterization of the 2,6-Dimethylphenol Monooxygenase MpdAB and Evaluation of Its Potential in Vitamin E Precursor Synthesis. Applied and Environmental Microbiology. 2022;88(8):e0011022.
[6] Cheng, Minggen; Pei, Dongmei; He, Xiangrong; Liu, Yongchuang; Zhu, Pingping; Yan, Xin*; The operon encoding hydrolytic dehalogenation of 4-chlorobenzoate is transcriptionally regulated by the TetR-type repressor FcbR and its ligand 4-chlorobenzoyl coenzyme A, Applied and Environmental Microbiology, 2021, 87(6)：20.
[7] Liu, Yongchuang; He, Xiangrong; Zhu, Pingping; Cheng, Minggen; Hong, Qing; Yan, Xin*; pheSAG Based Rapid and Efficient Markerless Mutagenesis in Methylotuvimicrobium, Frontiers in Microbiology, 2020, 11：441.
[8] Ji, Junbin; Zhang, Yanting; Liu, Yongchuang; Zhu, Pingping; Yan, Xin*; Biodegradation of plastic monomer 2,6-dimethylphenol by Mycobacterium neoaurum B5-4, Environmental Pollution, 2020, 258：113793.
[9] Leizhen Zhao, Bin Ye, Qi Zhang, Dan Cheng, Chaoyang Zhou, Shan Cheng, Xin Yan*. Construction of second generation protease‐deficient hosts of Bacillus subtilis for secretion of foreign proteins. Biotechnology and Bioengineering. 2019. DOI: 10.1002/bit.26992
[10] Zhou C, Ye B, Cheng S, Zhao L, Liu Y, Jiang J, Yan X*. Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis. Microb Cell Fact. 2019. 18(1):111. doi: 10.1186/s12934-019-1159-0.
[11] Xin Yan#, Junwei Huang#, Xihui Xu, Dian Chen, Xiangting Xie, Qing Tao, Jian He, Jiandong Jiang*. Enhanced and Complete Removal of Phenylurea Herbicides by Combinational Transgenic Plant-Microbe Remediation. Applied and Environmental Microbiology, 2018, 84:e00273-18. (Spotlight article)
[12] Xin Yan, Wen Jin, Guang Wu, Wankui Jiang, Zhangong Yang, Jumbin Ji, Jiguo Qiu, Jian He, Jiandong Jiang, Qing Hong*. Hydrolase CehA and Monooxygenase CfdC Are Responsible for Carbofuran Degradation in Sphingomonas sp. Strain CDS-1. Applied and Environmental Microbiology, 2018, DOI:10.1128/AEM.00805-18.
[13] Xueting Chen, Junbin Ji, Leizhen Zhao, Weiwu Wang, Jian He, Jiandong Jiang, Qing Hong, Xin Yan*. Molecular mechanism and genetic determinants of buprofezin degradation. Applied and Environmental Microbiology, 2017, 83:e00868-17. (Spotlight article)
[14] Minggen Cheng#, Qiang Meng#, Youjian Yang#, Cuiwei Chu, Qing Chen, Yi Li, Dan Cheng, Qing Hong, Xin Yan*, Jian He. The Two-Component Monooxygenase MeaXY Initiates the Downstream Pathway of Chloroacetanilide Herbicide Catabolism in Sphingomonads. Applied and Environmental Microbiology, 2017, 83(7): e03241-16. (Spotlight article)
[15] Xin Yan, Tao Gu, Zhongquan Yi, Junwei Huang, Xiaowei Liu, Ji Zhang, Xihui Xu, Zhihong Xin, Qing Hong, Jian He, Jim C. Spain, Shunpeng Li, Jiandong Jiang*. Comparative Genomic Analysis of Isoproturon-mineralizing sphingomonads Reveals the Isoproturon Catabolic Mechanism. Environmental Microbiology, 2016, 18(12): 4888-4906.
[16] Xin Yan*, Frances Chu, Aaron W. Puri, Yanfen Fu and Mary E. Lidstrom. Electroporation-Based Genetic Manipulation In Type I Methanotrophs. Applied and Environmental Microbiology, 2016, 82:7 2062-2069.
[17] Tao Gu, Chaoyang Zhou, Sebastian R. Sørensen, Ji Zhang, Jian He, Peiwen Yu, Xin Yan* and Shunpeng Li. The Novel Bacterial N-Demethylase PdmAB Is Responsible for the Initial Step of N,N-Dimethyl-Substituted Phenylurea Herbicide Degradation. Applied and Environmental Microbiology, 2013, 79(24): 7846-7856.
[18] Xin Yan, Hao-Jie Yu, Qing Hong, and Shun-Peng Li*. Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis. Applied and Environmental Microbiology, 2008, 74(17): 5556-5562.
